cell culture conditions human hek293t embryonic kidney cells  (ATCC)

Journal: Nature communications

Article Title: CoCas9 is a compact nuclease from the human microbiome for efficient and precise genome editing.

doi: 10.1038/s41467-024-47800-9

Figure Lengend Snippet: Fig. 5 | CoCas9 is active in human primary cells and the murine retina. Editing efficiency (% indels) of CoCas9 in 6 genomic loci of hematopoietic stem/progenitor cells (HSPCs) (representative experiment) (a), bronchial epithelial cells (HBE) (b) and skin fibroblasts (HSFs) (c) after transduction with lentiviral vectors and pur- omycin selection. d Frequency of A > G transitions at the HEKsite2 locus in HEK293T cells transduced with AAV-v1 and AAV-v2 (7 × 105 genome copies/cell, gc/ cell). e Percentages of indels at the RHO locus generated by AAV-v3 and AAV-v4

Article Snippet: Cell lines, primary human cells and culture conditions HEK293T cells (obtained from ATCC, #CRL-11268), U2OS.EGFP cells (a kind gift of Claudio Mussolino, University of Freiburg), harboring a single integrated copy of an EGFP reporter gene, AAVpro-293T (obtained from Takara, #632273), HEK293-RHO-EGFP cells and HSF (from the Coriell Institute, GM05659) were cultured in DMEM (Life Technologies) supplemented with 10% FBS (Life Technologies), 2mM GlutaMax (Life Technologies) and penicillin/streptomycin (Life Technologies).

Techniques: Transduction, Selection, Generated